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| Catalog Code: | NB110-66657 |
| Product Name: | FAK Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-FAK (EP841Y) [Tyr861] |
| Clone: | EP841Y |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Immunogen: | A synthetic peptide corresponding to residues surrounding tyrosine 861 of human FAK was used as an immunogen. |
| Specificity: | This antibody detects tyrosine 861 of human FAK. |
| Cross Reactivity: | This antibody is reactive against Human, Rat, and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody is useful for Western Blot. |
| Background: | Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Tyrosine 861 on FAK is a major Src phosphorylation site. Phospholyation on tyrosine 861 promotes FAK binding to Intergin (cytoplasmic tail) and leads to FAK mediated anti-apoptotic signaling pathway during hyperosmotic cell stress (4) Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5). |
| Storage: | Aliquot and store at -20 °C. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | Western Blot 1:10000-1:50000, |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | Anti-FADK 1 antibody, Anti-FADK antibody, Anti-FAK 1 antibody, Anti-FAK1 antibody, Anti-Focal adhesion kinase 1 antibody, Anti-pp125FAK antibody, Anti-Protein tyrosine kinase 2 antibody, Anti-Protein Tyrosine Kinase Cytoplasmic antibody, Anti-PTK 2 antibody, Anti-PTK2 antibody, Anti-PTK2 protein tyrosine kinase 2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Tyr861 |
| General References: | 1. Schaller, M.D. (2001) Biochim. Biophys. Acta 1540, 1-21 2. Schaller, M.D., Borgman, C.A., Cobb, B.S., Vines, R.R., Reynolds, A.B. and Parsons, J.T. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5192-5196 3. Salazar, E.P. and Rozengurt, E. (1999) J. Biol. Chem. 274, 28371-28378 4. J. Adrian Lunn, Rodrigo Jacamo, Enrique Rozengurt, JBC 282(14):10370-10379, 2007 5. Owens L. V., Xu L. H., Craven R. J., Dent G. A., Weiner T. M., Kornberg L., Liu E. T., Cance W. G. Cancer Res., 55: 2752-2755, 1995 |
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