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| Catalog Code: | NB110-66647 |
| Product Name: | PTP1B Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-PTP1B (EP1839Y) [Ser352] |
| Clone: | EP1839Y |
| Clonality: | Monoclonal |
| ListPrice: | 320 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Serine 352 of human PTP1B were used as an immunogen. |
| Specificity: | The residues surrounding Serine 352 of human PTP1B |
| Cross Reactivity: | This antibody is reactive against Human. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody is useful for Western Blot, Immunohistochemistry, and Immunoprecipitation. |
| Background: | The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3). |
| Storage: | Aliquot and store at -20 °C. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry 1:100-1:250, immunoprecipitation 1:40, Western Blot 1:2500-1:5000, |
| Species Summary: | Hu, |
| Alternate Names: | Anti-Non receptor tyrosine phosphatase 1 antibody; Anti-Protein tyrosine phosphatase 1B antibody; Anti-Protein tyrosine phosphatase non receptor type 1 antibody; Anti-Protein tyrosine phosphatase placental antibody; Anti-PTP 1B antibody; Anti-PTPN 1 antibody; Anti-PTPN1 antibody; Anti-Tyrosine protein phosphatase non receptor type 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Ser352 |
| General References: | 1. Flint AJ, et al. EMBO J 12(5):1937-46, 1993 2. Frangioni JV, et al. Cell 68(3):545-60, 1992 3. Ravichandran LV, et al Mol Endocrinol 5(10):1768-80, 2001. |
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