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| Catalog Code: | NB110-62105 |
| Product Name: | Cytokeratin 16 Antibody |
| Product Description: | Rabbit Monoclonal anti-Cytokeratin 16 (EP1615Y) |
| Clone: | EP1615Y |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | A synthetic peptide corresponding to residues near the N-terminus of human CK-16 was used as an immunogen. |
| Cross Reactivity: | This antibody is reactive against Human, Mouse, and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody is useful for Western Blot, Immunohistochemistry, Immunocytochemistry, FACS, and Immunoprecipitation. |
| Background: | The human type I keratin 16 (CK16) is constitutively expressed in a number of complex epithelial tissues, including skin, but is better known for its induction under conditions favoring enhanced proliferation or abnormal differentiation, including wound healing, psoriasis, and cancer (1). CK16 is expressed in suprabasal interfollicular epidermis in wound healing and other pathological conditions associated with hyperproliferation, such as psoriasis and are induced when keratinocytes are cultured in vitro. However, CK16 is also constitutively expressed in normal suprabasal mucosal and palmoplantar keratinocytes (2). Pachyonychia congenita (PC) is a group of inherited ectodermal dysplasias, the characteristic phenotype being hypertrophic nail dystrophy. The PC-1 phenotype may be distinguished by the absence of the epidermal cysts found in PC-2, and it has been shown to be caused by mutations in either keratin K16 or its expression partner, the K6a isoform of K6. Mutations in K16 have also been shown to cause a milder related phenotype, focal non-epidermolytic palmoplantar keratoderma (3). |
| Storage: | Aliquot and store at -20C. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis 1:50, immunohistochemistry 1:100-1:250, immunoprecipitation 1:30, Western Blot 1:5000-1:10000, Immunocytochemistry 1:100-1:250, |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | Anti-CK 16 antibody; Anti-CK16 antibody; Anti-Cytokeratin16 antibody; Anti-Focal non epidermolytic palmoplantar keratoderma antibody; Anti-K 16 antibody; Anti-K16 antibody; Anti-K1CP antibody; Anti-Keratin 16 antibody; Anti-Keratin type I cytoskeletal 16 antibody; Anti-Keratin16 antibody; Anti-KRT 16 antibody; Anti-KRT16 antibody; Anti-KRT16A antibody; Anti-NEPPK antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Paladini RD, et al. Biochem Biophys Res Commun 215(2): 517-23, 1995. 2. Shamsher MK, et al. Hum Mol Genet 4(10):1875-81, 1995 3. Smith FJ, et al. BR J Dermatol 141(6):1010-6, 1999 |
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