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| Catalog Code: | NB110-62084 |
| Product Name: | CDK1 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-CDK1 [Thr14, Tyr15] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | The antibody was produced against a synthetic phosphopeptide derived from the region of the human cyclin-dependent kinase that contains threonine 14 and tyrosine 15. |
| Specificity: | Human, mouse and rat cdk1. Other cyclin-dependent kinases phosphorylated on homologous residues have not been tested. No reactivity was found to cdk4 or cdk6. |
| Cross Reactivity: | This antibody is reactive against Human, Mouse, and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody is useful for Western Blot and Immunoprecipitation. |
| Background: | Mammalian cyclin-dependent protein kinase 1 (cdk1, 34 kDa) - also known as cdc2 in yeast - is the catalytic portion of a cyclin/cdk complex. It is known to associate with both cyclins A and B. Upon activation, cdk1 induces cells to progress from the G2 phase of the cell cycle through mitosis. This occurs when threonine 14 and tyrosine 15 are dephosphorylated in the presence of the phosphatase Cdc25. This relieves the inhibition on cdk1 phosphorylated on threonine 161, allowing it to become active. Myt1 and wee1 act to re-phosphorylate threonine 14 and tyrosine 15, causing inactivation. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host Name: | Rabbit |
| Buffer: | Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH |
| Application Summary: | immunoprecipitation 1:100, Western Blot 1:1000, |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-CDK1 antibody, anti-DKFZp686L20222 antibody, anti-MGC111195 antibody, Anti-CDK-1 antibody, Anti-CDK 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Thr14/Tyr15 |
| General References: | 1. Paulsen, M.T., et al. (2006) The p53-targeting human phosphatase hCdc14A interacts with the Cdk1/cyclin B complex and is differentially expressed in human cancers. Mol. Cancer 5(1):25. 2. Shi, X., et al. (2006) Phosphorylation of STAT3 serine-727 by cyclin-dependent kinase 1 is critical for nocodazole-induced mitotic arrest. Biochem. 45(18):5857-5867. 3. Jin, J., et al. (2003) SCFâ-TRCP links Cdk1 signaling to degradation of the Cdc25A protein phosphatase. Genes Dev. 17(24):3062-3074. 4. Porter, L.A. and D.J. Donoghue (2003) Cyclin B1 and CDK1: nuclear localization and upstream regulators. Prog. Cell Cycle Res. 5:335-347. 5. Ubersax, J.A., et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-864. 6. Karaiskou, A., et al. (2001) Differential regulation of Cdc2 and Cdk2 by RINGO and cyclins. J. Biol. Chem. 276(38):36028-36034. 7. Takizawa, C.G. and D.O. Morgan (2000) Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C. Curr. Opin. Cell Biol. 12(6):658-665. 8. Poon, R.Y., et al. (1997) Identification of functional domains in the neuronal Cdk5 activator protein. J. Biol. Chem. 272(9):5703-5708. 9. Sebastian, B., et al. (1993) Cdc25M2 activation of cyclin-dependent kinases by dephosphorylation of threonine-14 and tyrosine-15. Proc. Nat'l. Acad. Sci. USA 90(8):3521-3524. |
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