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| Catalog Code: | NB110-62081 |
| Product Name: | Beta Catenin Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-Beta Catenin [Ser45] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human â-catenin that contains serine 45. The sequence is conserved in mouse, rat and frog. |
| Specificity: | Human â-catenin. Mouse, rat and frog â-catenin (100% homologous) have not been tested, but are expected to react. |
| Cross Reactivity: | This antibody is reactive against Human. Mouse, rat and frog â-catenin are 100% homologous, but not yet tested. Other species not yet tested. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody is useful for Western Blot. |
| Background: | beta-catenin (92 kDa) is a multifunctional protein involved in cell-cell adhesion, intracellular signaling, and gene transcription. beta-catenin plays a key role in the Wnt pathway which mediates numerous developmental processes. Deregulation of the Wnt pathway results in the accumulation of beta-catenin in the nucleus. Stabilizing mutations of several serine/threonine residues (more frequently of serine 45) have been found in multiple tumors. Phosphorylation of â-catenin on serine 45 by casein kinase I (CKI) primes its subsequent phosphorylation of residues 33, 37, and 41 by glycogen synthase-3 beta (GSK-3â), and results in its degradation. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host Name: | Rabbit |
| Buffer: | Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH |
| Application Summary: | Western Blot 1:1000, |
| Species Summary: | Hu, |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Ser45 |
| General References: | 1. Tsukamoto, T., et al. (2003) beta-Catenin mutations and nuclear accumulation during progression of rat stomach adenocarcinomas. Cancer Sci. 94(12):1046-1051. 2. Marin, O., et al. (2003) A noncanonical sequence phosphorylated by casein kinase 1 in beta-catenin may play a role in casein kinase 1 targeting of important signaling proteins. Proc. Nat'l. Acad. Sci. USA 100(18):10193-10200. 3. Van Noort, M., et al., (2002) Identification of two novel regulated serines in the N terminus of beta-catenin. Exp. Cell Res. 276(2):264-272. 4. Amit, S., et al. (2002) Axin-mediated CKI phosphorylation of beta-catenin at Ser 45: a molecular switch for the Wnt pathway. Genes Dev. 16(9):1066-1076. 5. Kusafuka, T., et al. (2002) Codon 45 of the beta-catenin gene, a specific mutational target site of Wilms' tumor. Int. J. Mol. Med. 10(4):395-399. 6. Li, H., et al. (2002) beta-Catenin signaling: therapeutic strategies in oncology. Cancer Biol. Ther. 1(6):621-625. 7. Lamberti, C., et al. (2001) Regulation of beta-catenin function by the IkappaB kinases. J. Biol. Chem. 276(45):42276-42286. 8. Jeng, Y.M., et al. (2000) Somatic mutations of beta-catenin play a crucial role in the tumorigenesis of sporadic hepatoblastoma. Cancer Lett. 152(1):45-51. |
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