| request information: |
|
| Catalog Code: | NB110-62079 |
| Product Name: | AMPK alpha 1/2 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-AMPK alpha 1/2 [Thr172] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human AMPK that contains threonine 172. The sequence is conserved in human, mouse and rat. |
| Specificity: | Human, mouse and rat. |
| Cross Reactivity: | This antibody is reactive against Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody is useful in Western Blot. |
| Background: | AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostatis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host Name: | Rabbit |
| Buffer: | Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), p |
| Application Summary: | Western Blot 1:1000, |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | Anti-5 AMP activated protein kinase catalytic alpha 1 chain antibody, Anti-5', AMP activated protein kinase catalytic subunit alpha 1 antibody, Anti-AAPK1 antibody, Anti-acetyl CoA carboxylase kinase antibody, Anti-AMPK 1 antibody, Anti-AMPK alpha 1 chain antibody, Anti-AMPK antibody, Anti-AMPK1 antibody, Anti-AMPKa1 antibody, Anti-HMG CoA reductase kinase antibody, Anti-hormone sensitive lipase kinase antibody, Anti-kinase AMPK alpha1 antibody, Anti-MGC33776 antibody, Anti-MGC57364 antibody, Anti-PRKAA 1 antibody, Anti-PRKAA1 antibody, Anti-Protein kinase AMP activated alpha 1 catalytic subunit antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Thr172 |
| General References: | 1. McCullough, L.D. et al. (2005) Pharmacological inhibition of AMP-activated protein kinase provides neuroprotection in stroke. J. Biol. Chem. 280(21):20493-20502. 2. Jorgensen, S.B. et al. (2004) The alpha2-5'AMP-activated protein kinase is a site 2 glycogen synthase kinase in skeletal muscle and is responsive to glucose loading. Diabetes 53(12):3074-3081. 3. Shaw, R.J. et al. (2004) The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress. Proc. Natl. Acad. Sci. USA. 101(10):3329-3335. 4. Zhou, M.H. et al. (2004) Activation of the AMP-activated protein kinase by the anti-diabetic drug metformin in vivo. Role of mitochondrial reactive nitrogen species. J. Biol. Chem. 279(42):43940-43951. 5. Woods, A. et al. (2003) Identification of phosphorylation sites in AMP-activated protein kinase(AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis. J. Biol. Chem. 2003 Aug 1;278(31):28434-28442. 6. Yin, W. et al. (2003) Role of AMP-activated protein kinase in cyclic AMP-dependent lipolysis in 3T3-L1 adipocytes. J. Biol. Chem. 2003 Oct 31;278(44):43074-43080. Nagata, et al. (2003) AMP-activated protein kinase (AMPK) signaling in endothelial cells is essential for angiogenesis in response to hypoxic stress. J. Biol. Chem. 278(33):31000-31006. 7. Hawley, S.A. et al. (2002) The antidiabetic drug metformin activates the AMP-activated protein kinase cascade via an adenine nucleotide-independent mechanism. Diabetes 51(8):2420-2425. |
order or
more information: | company product webpage |
|