| CatalogCode: | NB110-62074 |
| ProductName: | FLT3 Antibody |
| Product Description: | Rabbit Polyclonal anti-FLT3 [Tyr969] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FLT3 that contains tyrosine 969. This sequence is conserved in chimpanzee (100% homologous). |
| Specificity: | Human. This antibody cross reacts with EGF and PDGF receptor. |
| CrossReactivity: | This antibody is reactive against Human. Not yet tested in other species. |
| Packaging: | 0.2 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody is useful in Western Blot. |
| Control: | RS 4;11 cells treated with FL (100 ng/mL) for 10 minutes. |
| Background: | FLT3 (Fms-like tyrosine kinase 3; also known as FLK2, STK1 (stem cell tyrosine kinase 1) or CD135) is a receptor tyrosine kinase for the hematopoietic growth factor FLT3 ligand (FL) and is expressed on both hematopoietic stem cells and progenitors. FLT3 belongs to the class III family of RTKs, which include c-Kit, CSF-R, and PDGFR. Activation of the receptor leads to tyrosine phosphorylation of a series of key adaptor proteins such as, Shc, Vav, PLCã, Gap, Dok, and p190RhoGAP. The sequence surrounding pY969 is a putative Grb2 binding site. FLT3 plays an important role in the proliferation, differentiation and survival of hematopoietic cells. Activating mutations of this receptor are the most common genetic abnormality seen in acute myeloid leukemia (AML). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| Buffer: | Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | Anti-CD 135 antibody; Anti-CD135 antigen antibody; Anti-Fetal liver kinase 2 antibody; Anti-FL cytokine receptor antibody; Anti-Flk 2 antibody; Anti-Flk2 antibody; Anti-Flt 3 antibody; Anti-Flt3 antibody; Anti-FMS like tyrosine kinase 3 antibody; Anti-Fms related tyrosine kinase 3 antibody; Anti-Growth factor receptor tyrosine kinase type III antibody; Anti-OTTHUMP0000004234 antibody; Anti-Stem cell tyrosine kinase 1 antibody; Anti-Stk 1 antibody; Anti-Stk1 antibody; Anti-Tyrosine protein kinase receptor FLT3 antibody |
| PackageSize: | 0.2 ml |
| GeneralRef: | 1. Brown, P., et al. (2005) FLT3 inhibition selectively kills childhood acute lymphoblastic leukemia cells with high levels of FLT3 expression. Blood 105(2):812-820. 2. Clark, J.J., et al. (2004) Variable sensitivity of FLT3 activation loop mutations to the small molecule tyrosine kinase inhibitor MLN518. Blood 104(9):2867-2872. 3. Drexler, H.G. and H. Quentmeier (2004) FLT3: Receptor and ligand. Growth Factors 22(2):71-73. 4. Schmidt-Arras, D., et al. (2004) Flt3 receptor tyrosine kinase as a drug target in leukemia. Curr. Pharm. Des. 10(16):1867-1883. 5. Yao, Q., et al. (2003) FLT3 expressing leukemias are selectively sensitive to inhibitors of the molecular chaperone heat shock protein 90 through destabilization of signal transduction-associated kinases. Clin. Cancer Res. 9:4483-4493. 6. Yee, K.W.H., et al. (2003) SU5416 and SU5414 inhibit kinase activity of wild-type and mutant FLT3 receptor tyrosine kinase. Blood 100(8):2941-2949. 7. Tse, K.F., et al. (2002) Inhibition of the transforming activity of FLT3 internal tandem duplication mutants from AML patients by a tyrosine kinase inhibitor. Leukemia 16(10):2027-2036. 8. Beslu, N., et al. (1996) Phosphatidylinositol-3' kinase is not required for mitogenesis or internalization of the Flt3/Flk2 receptor tyrosine kinase. J. Biol. Chem. 271(33):20075-20081. |
| Phosphorylated: | Tyr969 |
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