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| Catalog Code: | NB110-62072 |
| Product Name: | MTOR Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-MTOR [Ser2448] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human mTOR that contains serine 2448. The sequence is conserved in human, mouse and rat. |
| Specificity: | Human, mouse and hamster mTOR |
| Cross Reactivity: | This antibody is reactive in Human, Mouse, and Hamster. Rat is 100% homologus, but not yet tested. Other species not yet tested. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody is useful in Western Blot. |
| Background: | Mammalian target of Rapamycin (mTOR), also known as FKBP12-rapamycin-associated protein (FRAP) is a ~280 kDa serine/threonine kinase and a key modulator of cell growth and protein synthesis. mTOR is implicated in cancer and neurite plasticity, and plays a central role in mediating phosphoinositide 3-kinase (PI3 kinase) and Akt/PKB signals for cell growth, proliferation, and protein translation via ribosomal S6 kinase (S6Ks) and translation regulator eIF4E-binding protein 1. mTOR is differentially phosphorylated on threonine 2446 and serine 2448 in response to nutrient status and growth factor stimulation. Serine 2448 is located in the catalytic domain and is phosphorylated by Akt/PKB and in response to insulin, glucagons and amino acid stimulation. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host Name: | Rabbit |
| Buffer: | Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH |
| Application Summary: | Western Blot 1:1000, |
| Species Summary: | Ha, Hu, Mu, |
| Alternate Names: | Anti-FK506 binding protein 12 rapamycin complex associated protein 1 antibody; Anti-FK506 binding protein12-rapamycin associated protein 1 antibody; Anti-FK506 binding protein12-rapamycin associated protein 2 antibody; Anti-FKBP12 rapamycin complex associated protein antibody; Anti-FLJ44809 antibody; Anti-FRAP antibody; Anti-FRAP1 antibody; Anti-FRAP2 antibody; Anti-Mammalian target of rapamycin antibody; Anti-RAFT1 antibody; Anti-Rapamycin target protein antibody; Anti-RAPT1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Ser2448 |
| General References: | 1. Takahashi, K., et al. (2005) Differential membrane localization of ERas and Rheb, two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway. J. Biol. Chem. 280(38):32768-32774. 2. Tee, A.R. and J. Blenis (2005) mTOR, translational control and human disease. Semin. Cell Dev. Biol. 6(1):29-37. 3. Mourani, P.M., et al. (2004) Unique, highly proliferative growth phenotype expressed by embryonic and neointimal smooth muscle cells is driven by constitutive Akt, mTOR, and p70S6K signaling and is actively repressed by PTEN. Circulation 109(10):1299-1306. 4. Rao, R.D., et al. (2004) Mammalian target of rapamycin (mTOR) inhibitors as anti-cancer agents.Curr. Cancer Drug Targets 4(8):621-635. 5. Reynolds, T.H., et al. (2002) Control of Ser2448 phosphorylation in the mammalian target of rapamycin by insulin and skeletal muscle load. J. Biol. Chem. 277(20):17657-17662. 6. Zhang, X., et al. (2002) Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture. J. Biol. Chem. 277(31):28127-28134. Tang, S.J., et al. (2002) A rapamycin-sensitive signaling pathway contributes to long-term synaptic plasticity in the hippocampus. Proc. Nat'l. Acad. Sci. 99(1):467-472. |
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