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| Catalog Code: | NB110-61678 |
| Product Name: | SQSTM1 Antibody |
| Product Description: | Sheep Polyclonal anti-SQSTM1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide as part of human Ubiquitin Interactive Motif (UIM) derived from p62 protein, also known as SQSTM1. (accession:AAH01874) |
| Specificity: | Confirmed by immunohistochemical/ western blot analysis of normal and diseased human brain. Western blot: this antibody recognizes 4 major bands of 130, 62, 45, and 22 kDa in human brain homogenater. There are also some conserved SQSTM1 domains that are present in other species and recognized by this antibody. 80, 62 and 47 kDa proteins are identified in rat sypathetic ganglia. |
| Cross Reactivity: | This antibody is reactive against human. Not yet tested in other species. |
| Packaging: | 0.02 mg Affinity purified Sheep antisera. |
| Uses: | Western Blot and Immunohistochemistry. |
| Background: | SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. More recently, a role for SQSTM1/p62 in macroautophagic removal of intracellular protein aggregates has also been proposed. Studies involving the cellular depletion of SQSTM1/p62 have indicated a need for its association with LC3 and the aggregate proteins in order to facilitate correct formation of the autophagosome. In western blot the antibody recognizes 4 major bands of 130, 62, 45 and 22 kDa in human brain homogenate. The antibody may also recognize some proteins containing SQSTM1 domains from other species as 80, 62 and 47 kDa proteins are identified in rat sympathetic ganglia. Preincubation of the antibody with the immunizing peptide abolishes all reactivity in both human brain homogenate and rat sympathitic ganglia. |
| Storage: | Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host Name: | Sheep |
| Application Summary: | immunohistochemistry 1-4 ug/ml, Western Blot , |
| Species Summary: | Hu, |
| Alternate Names: | Anti-A170 antibody, Anti-EBI 3 associated protein of 60 kDa antibody, Anti-EBI3-associated protein of 60 kDa antibody, Anti-EBIAP antibody, Anti-ORCA antibody, Anti-OSIL antibody, Anti-Oxidative stress induced like antibody, Anti-p60 antibody, Anti-p62B antibody, Anti-Paget disease of bone 3 antibody, Anti-PDB 3 antibody, Anti-PDB3 antibody, Anti-Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody, Anti-Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody, Anti-Sequestosome-1 antibody, Anti-SQSTM 1 antibody, Anti-Ubiquitin-binding protein p62 antibody, Anti-ZIP 3 antibody, Anti-ZIP3 antibody |
| Package Size: | 0.02 mg |
| Preservative: | No Preservatives |
| General References: | Strausberg, R.L et al. Proc. Natl. Acad. Sci. U.S.A. 99(26) 16899-16903 (2002) |
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