| CatalogCode: | NB110-61671 |
| ProductName: | Ubiquitin Antibody |
| Product Description: | Sheep Polyclonal anti-Ubiquitin |
| Clonality: | Polyclonal |
| Immunogen: | Ubiquitin from bovine red blood cells coupled to chicken gamaclobulin boosted with ubiquitin. |
| Specificity: | Recognizes both free ubiquitin and ubiquitin protein conjugates on western blot. |
| CrossReactivity: | This antibody is reactive against human, rabbit, and sheep. Not yet tested in other species. |
| Packaging: | 0.1 ml Whole antisera Sheep antisera. |
| Uses: | Immunoshitochemistry and western blot |
| Background: | Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies. |
| Storage: | Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Host_Name: | Sheep |
| ListPrice: | 285 |
| AppSummary: | IHC, WB |
| SpeciesSummary: | Hu, Rb, Sh |
| ALTnames: | Anti-FLJ25987 antibody; Anti-MGC8385 antibody; Anti-Polyubiquitin B antibody; Anti-RPS27A antibody; Anti-UBA52 antibody; Anti-UBA80 antibody; Anti-UBB antibody; Anti-UBC antibody; Anti-UBCEP1 antibody; Anti-UBCEP2 antibody; Anti-Ubiquitin antibody; Anti-ubiquitin B antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | Haas et al (1985) J. Biol. Chem. 260:12464-12474 |
| NotesMain: | Reconstitute with addition of 0.1 ml of sterile water. |