| CatalogCode: | NB110-61586 |
| ProductName: | NHE3 Antibody |
| Product Description: | Mouse Monoclonal anti-NHE3 (19F5) |
| Clone: | 19F5 |
| Clonality: | Monoclonal |
| Immunogen: | Fusion protein representing the C-terminal 131 amino acids of rabbit NHE3. |
| Specificity: | This antibody does not show any cross-reactivity with any other NHE isoforms. |
| CrossReactivity: | Rat. Expected to react with rabbit. Does not react with human. Other species have not been tested. |
| Packaging: | 0.1 ml protein G purified Mouse ascites. |
| Uses: | This antibody is useful for Western blot analysis where a band can be seen at ~93 kDa. Though this production has not been tested, it is expected to be useful for immunoprecipitation, immunocytochemistry, and immunofluorescence. |
| Localization: | Membrane; multi-pass membrane. |
| Control: | Rat kidney membrane |
| Background: | Na+/H+ exchangers-1-8 (also designated NHE-1-8 or Na+/H+ antiporters) are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells. |
| ProductRef: | 1. Biemesderfer, D. et al Am. J. Physiol. 273(2 Pt 2):F289-99 (1997) 2. Kocinsky, H. Am. J. Physiol. Renal. 289: F249-F258 (2005) |
| Storage: | Store at 4C for short-term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Tris-glycine, 150mM NaCl |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu(-), Rt |
| ALTnames: | anti-Sodium/hydrogen exchanger 3 antibody, anti-Na (+)/H(+) exchanger 3 antibody, anti-NHE-3 antibody, anti-Solute carrier family 9 member 3 antibody, anti-SLC9A3 antibody |
| PackageSize: | 0.1 ml |