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| Catalog Code: | NB110-60562 |
| Product Name: | BNIP3 Antibody |
| Product Description: | Rabbit Polyclonal anti-BNIP3 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Gene ID: | 664 |
| Concentration: | 1 mg/ml |
| Immunogen: | A synthetic peptide against an N-terminal region (within residues 1-50) of the human BNIP3 protein. [Uniprot# Q12983] |
| Cross Reactivity: | Human. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody is useful for Western blot analysis where a band is seen in hypoxic samples at ~30 and 60 kDa, representing the BNIP3 protein. There are some non-specific bands present, as well. It is recommended that normoxic samples are run next to the hypoxic samples, as a negative control. |
| Localization: | Mitochondrion. Note: Co-expression with the EIB 19-kDa protein results in a shift in NIP3 localization to the nuclear envelope. |
| Background: | BNIP3, formerly NIP3 (nineteen kDa interacting protein-3), is a pro-apoptotic, mitochondrial protein. It represents a subfamily of Bcl 2 related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and non-mitochondrial sites by selective Bcl 2/Bcl XL interactions. It interacts with the E1B 19 kDa protein, which is responsible for the protection of virally-induced cell death, and the E1B 19 kDa-like sequence of Bcl2, also an apoptotic protector. BNIP3 also plays a role in autophagy. Hypoxia-induced autophagy requires expression of this protein. Beclin, an autophagy protein, is kept in check by Bcl2 (normoxic). When Bcl2 lets go of Beclin it then binds BNIP3/L3 (hypoxic), causing autophagy. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Host Name: | Rabbit |
| Buffer: | Tris-citrate/phosphate [pH 7-8] |
| Application Summary: | Western Blot 1:500 - 1:1,000 |
| Species Summary: | Hu, |
| Alternate Names: | anti-BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.09% Sodium Azide |
| Homology: | 100% homology with human protein. 93% homology with cat protein. 87% homology with cow and dog proteins. |
| General References: | 1. Zhang, L. et al. Free Radic. Biol. Med. 43(1): 117-127 (2007) |
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