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| Catalog Code: | NB110-60531SS |
| Product Name: | ApoE Antibody |
| Product Description: | Mouse Monoclonal anti-ApoE (WUE-4), Sample Size |
| Clone: | WUE-4 |
| Clonality: | Monoclonal |
| ListPrice: | 100 |
| Gene ID: | 348 |
| Concentration: | 0.85 mg/ml |
| Immunogen: | Purified human ApoE. [Uniprot# P02649] |
| Epitope: | Residues 140-160 of purified human ApoE (LDLR receptor binding site). |
| Cross Reactivity: | Human. Though not tested, it is likely to react with mouse. It does not appear to react with rat brain tissue. Other species have not been tested. |
| Packaging: | 0.025 ml protein G purified Mouse ascites. |
| Uses: | This antibody is useful for Western blot analysis where a band is seen at ~36 kDa, representing the ApoE protein. It is also useful for ELISA. Though this production has not been tested, other productions of this antibody have been useful for immunoprecipitation and immunohistochemistry. |
| Localization: | Secreted |
| Background: | Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes. Apolipoprotein E is a type of lipoprotein. Apolipoprotein E is associated with several cardiovascular disorders. There are three isoforms (slightly different forms) of the ApoE lipoprotein. These three isoforms are known as ApoE2, E3 and E4. ApoE2 seems to be one of several genetic factors that plays a part in increased risk of heart attacks and strokes and ApoE4 is a risk factor for coronary artery disease. As well, ApoE4 has been been suggested to play a role in type 2 Alzheimer disease. |
| Storage: | Store at 4C for short term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 kappa |
| Host Name: | Mouse |
| Buffer: | Tris-glycine, 150mM NaCl |
| Application Summary: | ELISA , Western Blot 2 ug/ml |
| Species Summary: | Hu, |
| Alternate Names: | anti-Apolipoprotein E antibody, anti-Apo-E antibody |
| Package Size: | 0.025 ml |
| Preservative: | 0.05% sodium azide |
| General References: | 1. Wahrle, SE. et al. JBC. 280: 43236-43242 (2005) |
| Product References: | 1. Krul, ES., et al. J. Lipid Research. 29: 1309-1325 (1988) [Western blot] 2. Fryer, JD., et al. JBC. 280: 25754-25759 (2005) [ELISA, Immunohistochemistry] |
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more information: | company product webpage |