| CatalogCode: | NB110-60021 |
| ProductName: | CD147 Antibody |
| Product Description: | Mouse Monoclonal anti-CD147 (MEM-M6/6) |
| Clone: | MEM-M6/6 |
| Clonality: | Monoclonal |
| Immunogen: | Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimmer). |
| Specificity: | The antibody MEM-M6/6 recognizes Ig domain D2 (membrane proximal) of CD147(Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by restinglymphocytes and red blood cells. |
| CrossReactivity: | Species Reactivity: Human |
| Packaging: | protein A purified Mouse ascites. |
| Uses: | Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.Application note: Non-reducing conditions.Functional ApplicationThe antibody MEM-M6/6 (high-affinity mAb of unique epitope specificity) inhibits anti-CD3-induced T cell activation. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 |
| ListPrice: | 285 |
| AppSummary: | FACS, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-extracellular matrix metalloproteinase inducer antibody; anti-tcsf antibody; anti-emmprin ok blood group antibody; anti-collagenase stimulatory factor antibody; anti-basigin ok blood group antibody; anti-basigin; 5f7 antibody; anti-m6 antigen antibody; anti-basigin antibody |
| GeneralRef: | *Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H.: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86. |