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| Catalog Code: | NB110-60014 |
| Product Name: | PDCD4 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-PDCD4 [Ser457] |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.95 mg/ml |
| Immunogen: | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein. |
| Specificity: | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. |
| Cross Reactivity: | Reactive with human, mouse, rat and xenopus, based on 100% homology with immunogen. |
| Packaging: | 0.1 mg Affinity purified Rabbit antisera. |
| Uses: | This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 52kDa in size corresponding to Pdcd4 protein is expected in the appropriate cell lysate or extract. |
| Background: | Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1 responsive promoters by c-Jun. |
| Purity: | Affinity purified |
| Host Name: | Rabbit |
| Buffer: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Application Summary: | ELISA 1:400, immunoprecipitation , Western Blot 1:500 - 1:2000 |
| Species Summary: | Hu, Mu, Rt, Xp, |
| Package Size: | 0.1 mg |
| Preservative: | 0.01% Sodium Azide |
| Phosphorylated: | Ser457 |
| General References: | 1. Jansen AP, Camalier CE, Colburn NH (2005) Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis. Cancer Res. 65(14):6034-6041. 2. Palamarchuk A, Efanov A, Maximov V, Aqeilan RI, Croce CM, Pekarsky Y (2005) Akt phosphorylates and regulates Pdcd4 tumor suppressor protein. Cancer Res. 65 (24):11282-11286. |
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