| CatalogCode: | NB110-60013 |
| ProductName: | PDCD4 Antibody |
| Product Description: | Rabbit Polyclonal anti-PDCD4 |
| Clonality: | Polyclonal |
| Immunogen: | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein. |
| Specificity: | This affinity purified antibody is directed against human Pdcd4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457. |
| CrossReactivity: | This antibody is reactive with human, mouse, rat and xenopus, based on 100% homology with the antigen. |
| Packaging: | 0.1 mg Affinity purified Rabbit antisera. |
| Uses: | This affinity purified antibody has been tested for use in ELISA, western blotting and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Pdcd4 protein by western blotting in the appropriate cell lysate or extract. |
| Background: | Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Host_Name: | Rabbit |
| Buffer: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| ListPrice: | 295 |
| AppSummary: | ELISA, IHC, WB |
| SpeciesSummary: | Hu, Mu, Rt, Xp |
| ALTnames: | anti-H731 antibody, anti-MGC33046 antibody, anti-MGC33047 antibody, anti-Neoplastic transformation inhibitor antibody, anti-Neoplastic transformation inhibitor protein antibody, anti-Nuclear antigen H731 antibody, anti-Nuclear antigen H731 like antibody, anti-Nuclear antigen H731 like protein antibody, anti-PDCD 4 antibody, anti-Programmed cell death 4 antibody, anti-Programmed cell death protein 4 antibody, anti-Protein 197/15a antibody, anti-RP11 348N5.4 antibody |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Jansen AP, Camalier CE, Colburn NH (2005) Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis. Cancer Res. 65 (14): 6034-6041. 2. Palamarchuk A, Efanov A, Maximov V, Aqeilan RI, Croce CM, Pekarsky Y (2005) Akt phosphorylates and regulates Pdcd4 tumor suppressor protein. Cancer Res. 65 (24): 11282-11286. |
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