| CatalogCode: | | NB110-59979 |
| ProductName: | | P2X3 Antibody |
| Product Description: | | Guinea Pig Monoclonal anti-P2X3 |
| Clonality: | | Monoclonal |
| Immunogen: | | VEKQSTDSGAYSIGH Corresponding to residues 383-397 of the carboxy-terminus of rat P2X3 |
| CrossReactivity: | | This antibody is reactive against rat. |
| Packaging: | | 0.1 ml Whole antisera Guinea Pig ascites. |
| Uses: | | Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. |
| Background: | | P2X3 is a subunit in the family of ion channels activated by extracellular ATP. It was cloned from rat dorsal root ganglia. P2X3 has two putative transmembrane domains with intracellular N- and C-termini. It can form homomeric channels and heteromeric channels with other members of the family. P2X3 is expressed in nociceptive sensory neurons |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Whole antisera |
| Isotype: | | IgG |
| Host_Name: | | Guinea Pig |
| ListPrice: | | 295 |
| AppSummary: | | IHC |
| SpeciesSummary: | | Rt |
| PackageSize: | | 0.1 ml |
| more information: | | company product webpage |