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| Catalog Code: | NB110-59978 |
| Product Name: | NPY Y2 Receptor Antibody |
| Product Description: | Rabbit Polyclonal anti-NPY Y2 Receptor |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1.28 mg/ml |
| Immunogen: | (C)TDSFSEATNV. Cys added for conjugation to KLH |
| Cross Reactivity: | This antibody is reactive against mouse and rat. |
| Packaging: | 0.1 ml Affinity purified Rabbit antisera. |
| Uses: | This antibody works in IHC. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS.Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. |
| Background: | This NPY Y2 antibody has been extensively characterized at the spinal level and is currently being characerized in brain. The antibody has been tested in both rat and mouse and appears to work better on mose tissue, although positive staining is seen in rat. For full characterizarion in dorsal root ganglion and spinal cord. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry 1:500 -1:1000 |
| Species Summary: | Mu, Rt, |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% sodium azide |
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more information: | company product webpage |