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| Catalog Code: | NB110-59938 |
| Product Name: | Catalase Antibody |
| Product Description: | Rabbit Monoclonal anti-Catalase (EP1929Y) |
| Clone: | EP1929Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues near the C-terminus of human Catalase was used as an immunogen. |
| Cross Reactivity: | This antibody is reactive against human, mouse and rat. |
| Packaging: | 0.1 ml |
| Uses: | This antibody works in WB, IHC, ICC, FC. The molecular weight in WB is 60 kDa |
| Background: | Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | immunohistochemistry 1:100 250, Western Blot 1:10,000 20,000, Immunocytochemistry 1:100 250, FC 1:50 |
| Species Summary: | Hu, Mu, Rt, |
| Package Size: | 0.1 ml |
| General References: | 1. Putnam CD, et al. J Mol Biol 296(1):295-309, 2000 2. Jin LH, et al. Free Radic Biol Med 31(11):1509-19, 2001 3. Agar NS, et al. J Clin Invest 77(1):319, 1986 |
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