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| Catalog Code: | NB110-59722SS |
| Product Name: | CIP2A Antibody |
| Product Description: | Mouse Monoclonal anti-CIP2A (2G10), Sample Size |
| Clone: | 2G10 |
| Clonality: | Monoclonal |
| ListPrice: | 100 |
| Concentration: | 1.8 mg/ml |
| Immunogen: | A C-terminal portion of the human p90 protein. |
| Epitope: | A 300 amino acid portion of the C-terminus. |
| Cross Reactivity: | This antibody reacts with the human protein. Other species have not been tested. |
| Packaging: | 0.025 ml protein G purified Mouse ascites. |
| Uses: | This antibody can be used for Western blot analysis where a band is seen at ~90 kDa. This antibody may also be useful for immunofluorescence. |
| Localization: | Cytoplasmic |
| Background: | Inhibition of protein phosphatase 2A (PP2A) activity has been identified as a prerequisite for the transformation of human cells. The protein, designated Cancerous Inhibitor of PP2A (CIP2A), interacts directly with the oncogenic transcription factor c-Myc, inhibits PP2A activity toward c-Myc serine 62 (S62), and thereby prevents c-Myc proteolytic degradation. In addition to its function in c-Myc stabilization, CIP2A promotes anchorage-independent cell growth and in vivo tumor formation. The oncogenic activity of CIP2A is demonstrated by transformation of human cells by overexpression of CIP2A. Importantly, CIP2A is overexpressed in two common human malignancies, head and neck squamous cell carcinoma (HNSCC) and colon cancer. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2b kappa |
| Host Name: | Mouse |
| Buffer: | Tris-glycine, 150mM NaCl |
| Application Summary: | immunofluorescence - Assay dependent, Western Blot - 0.5-2 ug/ml |
| Species Summary: | Hu |
| Alternate Names: | anti-Cancerous Inhibitor of Protein Phosphatase 2A antibody, anti-p90 antibody |
| Package Size: | 0.025 ml |
| Preservative: | 0.05% sodium azide |
| General References: | 1. Junttila, M.R., et al. CIP2A Inhibits PP2A in Human Malignancies. Cell. 130: 51-62 (2007) 2. Shi, F.D., et al. Preferential humoral immune response in prostate cancer to cellular proteins p90 and p62 in a panel of tumor associated antigens. Prostate. 63: 252-258. (2005) |
| Product References: | 1. Soo Hoo, L., et al. Cloning and characterization of a novel 90 kDa 'companion' auto-antigen of p62 overexpressed in cancer. Oncogene. 21: 5006-5015 (2002) |
| Web Link: | 1. Li, W., Ge, Z., Liu, C., et al. CIP2A is overexpressed in gastric cancer and its depletion leads to impaired clonogenicity, senescence, or differentiation of tumor cells. Clin. Cancer Res., Jun 2008; 14: 3722-3728. |
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