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| Catalog Code: | NB110-58875 |
| Product Name: | MARCKS Antibody |
| Product Description: | Rabbit Polyclonal anti-MARCKS |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Gene ID: | 4082 |
| Immunogen: | This antibody was made against full length recombinant MARCKS expressed in and purified from E. coli. |
| Specificity: | The MARCKS protein runs at about 80kDa on SDS-PAGE gels, although the real molecular weight is much lower, at about 31.5kDa. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein. |
| Cross Reactivity: | This antibody reacts with rat protein. Other species have not been tested. |
| Packaging: | 0.1 ml Whole Rabbit antisera. |
| Uses: | This antibody is useful for immunofluorescence, ABC or other enzyme linked immunocytochemical procedures, and Western blotting. |
| Background: | Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Host Name: | Rabbit |
| Application Summary: | immunohistochemistry 1:5000, immunofluorescence 1:500-1:1000, Western Blot 1:20000, Immunocytochemistry 1:5000 |
| Species Summary: | Rt |
| Alternate Names: | anti-Myristolyated alanine rich C-kinase substrate antibody, anti-MARCS antibody, anti-Protein kinase C substrate, 80 kDa protein, light chain antibody, anti-PKCSL 80K-L protein antibody, anti-MACS antibody, anti-PRKCSL antibody |
| Package Size: | 0.1 ml |
| Preservative: | No preservative |
| General References: | 1. Hirai, M. and Shimizu N. Purification of two distinct proteins of approximate Mr 80,000 from human epithelial cells and identification as proper substrates for protein kinase C. Biochem J. 270:583-9 (1990) 2. Hartwig, JH, et al. MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622 (1992) 3. Blackshear, PJ. The MARCKS family of cellular protein kinase C substrates. JBC. 268: 1501-1504 (1993) 4. Stumpo, DJ, et al. MARCKS deficiency in mice leads to abnormal brain development and perinatal death. PNAS. 92; 944-948 (1995) |
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