| request information: |
|
| Catalog Code: | NB110-58874 |
| Product Name: | UCHL1 Antibody |
| Product Description: | Rabbit Polyclonal anti-UCHL1 - Neuronal Marker |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Gene ID: | 7345 |
| Concentration: | 60 mg/ml |
| Immunogen: | This antibody was raised in rabbit against recombinant full length human UCHL1 purified from E. coli. |
| Cross Reactivity: | This antibody reacts with human and rat proteins. Other species have not been tested. |
| Packaging: | 0.1 ml Whole Rabbit antisera. |
| Uses: | This antibody is useful for immunofluorescence, ABC or other enzyme linked immunocytochemical procedures, and Western blotting. |
| Background: | Ubiquitin C-terminal hydrolase 1 (UCHL1) was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. The protein is extremely abundant representing 1-2% of total brain protein. It was later found that a ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein, resulting in the renaming of PGP9.5 to ubiquitin C-terminal hydrolase 1. This is the first of a family of ubiquitin C-terminal hydrolases which have been characterized, and is expressed heavily in neurons in the brain. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. This activity is important to generate mono-ubiquitin from genes which encode polyubiquitin chains or ubiquitin fused to other proteins. The activity is also important to remove ubiquitin from partially degraded proteins, allowing the ubiquitin monomer to be recycled. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Recent studies suggest that UCHL1 also has a ubiqutinyl ligase activity, being able to couple ubiquitin monomers by linking the C-terminus of one with lysine 63 of the other. Since UCHL1 is heavily expressed in neurons, antibodies to UCHL1 can be used to identify neurons in histological sections and in tissue culture. The great abundance of this protein in neurons means that it is released from neurons in large amounts following injury or degeneration, so the detection of of UCHL1 in CSF and other bodily fluids can be used as a biomarker. UCHL1 was also discovered as a gene mutated in some rare famial forms of Parkinson's disease. Interestingly a common allelic variant of UCHL1, the S18Y polymorphism is actually protective against Parkinson's disease. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Host Name: | Rabbit |
| Application Summary: | immunofluorescence 1:1000, Western Blot 1:10000 |
| Species Summary: | Hu, Rt, |
| Alternate Names: | anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody, anti-EC 3.4.19.12 antibody, anti-UCH-L1 antibody, anti-Ubiquitin thioesterase L1 antibody, anti-Neuron cytoplasmic protein 9.5 antibody, anti-PGP 9.5 antibody, anti-PGP9.5 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 10 mM sodium azide |
| Marker: | Neuronal Marker |
| General References: | 1. Doran JF, Jackson P, Kynoch PA, Thompson RJ. Isolation of PGP 9.5, a new human neurone-specific protein detected by high-resolution two-dimensional electrophoresis. J Neurochem. 40:1542-7 (1983). 2. Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J. The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase. Science. 1989 246:670-3 (1989). 3. Kurihara LJ, Kikuchi T, Wada K, Tilghman SM. Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia.Hum Mol Genet. 10:1963-70 (2001). 4. Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr. The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Cell 111:209-18 (2002). |
order or
more information: | company product webpage |
|