| request information: |
|
| Catalog Code: | NB110-58732 |
| Product Name: | CD54 Antibody |
| Product Description: | Mouse Monoclonal anti-CD54 (1H4) |
| Clone: | 1H4 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Raji cells and spleen cells fused with NS1 cells. |
| Specificity: | The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. |
| Cross Reactivity: | Human |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | For immunohistochemistry in paraffin sections prolonged fixation in buffered formalin will destroy the epitope. High-temperature antigen unmasking is recommended. |
| Background: | ICAM-1 (MW 90 kD) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG2b |
| Host Name: | Mouse |
| Buffer: | PBS, pH 7.4 |
| Application Summary: | flow cytometry / FACS analysis , immunohistochemistry -P 10-20ug/ml, -Fr 5-20ug/ml, immunoprecipitation , Immunocytochemistry , |
| Species Summary: | Hu, |
| Package Size: | 0.1 mg |
| Preservative: | No Preservative |
order or
more information: | company product webpage |
|