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| Catalog Code: | NB110-58727 |
| Product Name: | CD34 Antibody |
| Product Description: | Mouse Monoclonal anti-CD34 (4H11(APG)) |
| Clone: | 4H11(APG) |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia. |
| Specificity: | The antibody 4H11[APG] reacts with Class III epitope on CD34 (Mucosialin), a 110 kDa monomeric transmembrane phosphoglycoprotein with two extracellular domains. CD34 is expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line. |
| Cross Reactivity: | Human |
| Packaging: | 0.1 mg Affinity purified Mouse ascites. |
| Uses: | For Western Blot, use under non-reducing conditions. Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Negative Control: Jurkat human leukemia T-cell line. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS, pH 7.4 |
| Application Summary: | flow cytometry / FACS analysis , Western Blot , |
| Species Summary: | Hu, |
| Package Size: | 0.1 mg |
| Preservative: | Sodium Azide |
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