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| Catalog Code: | NB110-57658 |
| Product Name: | Caspase 9 Antibody |
| Product Description: | Rabbit Polyclonal anti-Caspase 9 |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Recombinant protein corresponding to aa 1-134 of human Caspase 9. |
| Specificity: | Detects a band at 46-48 kDa. |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Cow and Sheep. Not tested in other species. |
| Packaging: | 0.5 ml protein A purified Rabbit antisera. |
| Uses: | IP: Denatured Verified (use Protein A)IHC (Formalin/paraffin): staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0. |
| Localization: | Cytoplasmic |
| Background: | Activation of procaspase-9 by Apaf-1 in the cytochrome c/dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c and dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, caspase-9 can initiate a caspase cascade involving the downstream executioners caspase-3, -6, and -7. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Host Name: | Rabbit |
| Buffer: | 10mM PBS, pH 7.4 with 0.2% BSA |
| Application Summary: | immunoprecipitation 10ug/mg protein lysate, Western Blot 2.5-5.0 ug/ml for 2hrs at RT, Immunohistochemistry-Paraffin 5-10 ug/ml for 30 min at RT |
| Species Summary: | Bv, Hu, Mu, Rt, Sh, |
| Alternate Names: | anti-Caspase-9 antibody |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% sodium azide |
| General References: | 1. Li P, et al. Cell 1997, 91(4):479-89. 2. Hakem R, et al.Cell 1998, 94(3):339-52. |
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