| CatalogCode: | NB110-57654 |
| ProductName: | ZAP70 Antibody |
| Product Description: | Rabbit Monoclonal anti-ZAP70 (YE291) |
| Clone: | YE291 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues in C-terminus of human ZAP-70 was used as immunogen. |
| CrossReactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500IHC: 1-100-250ICC: 1:100-250Flow Cyrometry: 1:80IP: 1:50 |
| Background: | Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | 70 kDa zeta associated protein antibody Selective T cell defect antibody SRK antibody STD antibody Syk related tyrosine kinase antibody Truncated ZAP kinase antibody Tyrosine protein kinase ZAP70 antibody TZK antibody ZAP-70 antibody Zeta chain associated protein kinase 70kDa isoform 1 antibody Zeta chain associated protein kinase 70kDa isoform 2 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Chu, D.H., et al. The Syk family of protein tyrosine kinases in T-cell activation and development. Immunol. Rev. 165: 167-180 (1998). 2. Isakov, N., et al. ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity. J Exp Med. 181: 375-80 (1995). 3. Di Bartolo, V., et al. Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling. J Biol Chem. 274: 6285-94 (1999). |
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