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| Catalog Code: | NB110-57653 |
| Product Name: | ZAP-70 Antibody |
| Product Description: | Rabbit Monoclonal anti-ZAP-70 (E267) |
| Clone: | E267 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in N-terminus of human ZAP-70 was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:500Flow Cyrometry: 1:40IP: 1:30 |
| Background: | Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-70 kDa zeta associated protein antibody, anti-Selective T cell defect antibody, anti-SRK antibody,anti-TD antibody, anti-Syk related tyrosine kinase antibody, anti-Truncated ZAP kinase antibody, anti-Tyrosine protein kinase ZAP70 antibody, anti-TZK antibody, anti-ZAP-70 antibody, anti-Zeta chain associated protein kinase 70kDa isoform 1 antibody, anti-Zeta chain associated protein kinase 70kDa isoform 2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Chu, D.H., et al. The Syk family of protein tyrosine kinases in T-cell activation and development. Immunol. Rev. 165: 167-180 (1998). 2. Isakov, N., et al. ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity. J Exp Med. 181: 375-80 (1995). 3. Di Bartolo, V., et al. Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling. J Biol Chem. 274: 6285-94 (1999). |
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