| request information: |
|
| Catalog Code: | NB110-57647 |
| Product Name: | Vimentin Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Vimentin Phosphospecific (EP1070Y) [Ser72] |
| Clone: | EP1070Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic acetylated peptide corresponding to residues surrounding Ser72 of Vimentin was used as an immunogen. The antibody only detects Vimentin phosphorylated at Serine 72. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1,000,000IP: 1:40 |
| Background: | Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed in during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-FLJ36605 antibody, anti-VIM antibody, anti-vimentin antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Ser72 |
| General References: | 1. Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992. 2. Inagaki M et al. Nature 328: 649-652, 1987. 3. Goto H et al. GENES CELLS 7:91-97. 2002. 4. Goto H et al. J Biol Chem, 273(19):11728-11736, 1998. 5. Yamaguchi T et al. JCB, 171(3):431-436, 2005. |
order or
more information: | company product webpage |
|