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| Catalog Code: | NB110-57642 |
| Product Name: | VEGF (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-VEGF (C-term) (EP1176Y) |
| Clone: | EP1176Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues on the C-terminus of human VEGF was used as an immunogen. |
| Cross Reactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1000IHC: 1:50 - 100ICC: 1:250 - 500FC: 1:25IP: 1:50 |
| Background: | Vascular endothelial growth factor (VEGF) was recently identified as a secreted, direct-acting mitogen specific for vascular endothelial cells and capable of stimulating angiogenesis in vivo. Molecular cloning revealed multiple forms of VEGF, apparently arising from alternative splicing of its RNA transcript (1). VEGF and TGF-beta1 are potent angiogenesis inducers with opposing effects on endothelial cells. TGF-beta1 induces apoptosis; VEGF protects endothelial cells from apoptosis. We found that TGF-beta1 promotes endothelial cell expression of FGF-2, which up-regulates VEGF synthesis. Inhibition of VEGF signaling through VEGF receptor 2 (flk-1) abrogates TGF-beta1-induced apoptosis and p38(MAPK) activation. This finding illustrates an unexpected role of VEGF and indicates that VEGF can be pharmacologically converted into an apoptotic factor, a novel approach to antiangiogenesis therapy (2). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-MGC70609 antibody, anti-Vascular Endothelial Growth Factor antibody, anti-Vascular Permeability Factor antibody, anti-VEGF 121 antibody, anti-VEGFA antibody, anti-VPF antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Houck KA, et al. Mol Endocrinol 5(12):1806-14, 1991. 2. Ferrari G, et al. Proc Natl Acad Sci USA 103(46):17260-5, 2006. |
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