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| Catalog Code: | NB110-57638 |
| Product Name: | Ubiquitin Antibody |
| Product Description: | Rabbit Monoclonal anti-Ubiquitin (EP296Y) |
| Clone: | EP296Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1,000IHC: 1:250-500 |
| Background: | Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-FLJ25987 antibody, anti-MGC8385 antibody, anti-Polyubiquitin B antibody, anti-RPS27A antibody, anti-UBA52 antibody, anti-UBA80 antibody, anti-UBB antibody, anti-UBC antibody, anti-UBCEP1 antibody, anti-UBCEP2 antibody, anti-Ubiquitin antibody, anti-ubiquitin B antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Goldstein,G., Scheid, M., Hamerling, U., Boyse, E.A., Schlesinger, D.H. & Niall, D. (1975) Proc. Natl. Acad. Sci. USA 72 1-15. 2. Hatfield, P.M. & Vierstra, R.D. (1992) J. Biol. Chem. 267 14799-14803. 3. Sullivan, M.L. & Vierstra, R.D. (1991) J. Biol. Chem. 266 23878-23885. 4. Gonen, H., et al. (1996) J Biol Chem 271 302-310. 5. Hatakeyama S. , Nakayama K.I. Biochem. Biophys. Res. Commun. 302: 635-645 (2003). |
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