| request information: |
|
| Catalog Code: | NB110-57636 |
| Product Name: | Tyrosinase (TYR) Antibody |
| Product Description: | Rabbit Monoclonal anti-Tyrosinase (TYR) (EP1577Y) |
| Clone: | EP1577Y |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Specificity: | A synthetic peptide corresponding to residues near the N-terminus of human Tyrosinase was used as an immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:2000IHC: 1:100 - 250FC: 1:15IP: 1:40 |
| Background: | Tyrosinase is a rate-limiting enzyme in the melanin biosynthetic pathway and a complete defect of the enzyme activity caused by homozygous mutations of the tyrosinase gene is well known to result in tyrosinase-negative oculocutaneous albinism (OCA1A) patients who never develop any melanin pigment in the skin, hair and eyes throughout life (1). Tyrosinase catalyzes the first two steps of melanin biosynthesis. Mutations of the TYR gene have been identified in a large number of patients, most of Caucasian ethnic origin, with various forms of type I OCA (2). Type I OCA results from mutations of the gene encoding tyrosinase, the enzyme that catalyzes the first 2 steps of melanin pigment biosynthesis. In type IA (tyrosinase-negative) OCA tyrosinase enzymatic activity is completely absent, and in type IB (yellow) OCA tyrosinase activity is greatly reduced. More than 80% of the known missense substitutions associated with type I OCA cluster within 2 relatively small regions of the tyrosinase polypeptide, suggesting that these may correspond to functionally important sites within the enzyme (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-LB24 AB antibody, anti-Monophenol monooxygenase antibody, anti-SK29 AB antibody, anti-Tumor rejection antigen AB antibody, anti-OCA1A antibody, anti-OCAIA antibody, anti-Oculocutaneous albinism IA antibody, anti-TYR antibody, anti-tyrosinase (oculocutaneous albinism IA) antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Tripathi RK, et al. Am J Med Genet 43(5):865-71, 1992. 2. Tripathi RK, et al. Am J Med Genet 53(6):1173-9, 1993. 3. Nakamura E, et al. J Dermatol Sci 28(2):102-5, 2002 |
order or
more information: | company product webpage |
|
|