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| Catalog Code: | NB110-57632 |
| Product Name: | TSC2/Tuberin Antibody |
| Product Description: | Rabbit Monoclonal anti-TSC2/Tuberin (Y320) |
| Clone: | Y320 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the C-term of human TSC2 was used as an immunogen. Predicted to detect isoform 2-4 based on sequence homology. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:200 - 1000IHC: 1:50Flow Cytometry: 1: 50 |
| Background: | Tuberous sclerosis complexe-2 (TCS2 also known as Tuberin) is a tumor suppressor that form a complex with TSC1 (Hamartin) and this complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1, Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Additionally, binding of 14-3-3 beta to TSC2 at Ser1210 reduce the ability of the complex to inhibit phosphorylation of S6 kinase (4). Phosphorylation by ATK at Ser924 and Thr1518 inactivates TSC2 and disrupts its interaction with TSC1 (5). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-FLJ43106 antibody, anti-LAM antibody, anti-TSC2 antibody, anti-TSC4 antibody, anti-Tuberin antibody, anti-Tuberous sclerosis 2 protein antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Lamb,et al. Nature Cell Biol. 2: 281-287 2. Xiao et al J. Biol. Chem. 272: 6097-6100 3. Manning, B. D.; Tee, A. R.; Logsdon, M. N.; Blenis, J.; Cantley, L. C. : Molec. Cell 10: 151-162, 2002. 4. Shumway, S. D.; Li, Y.; Xiong, Y. : J. Biol. Chem. 278: 2089-2092, 2003. 5. Inoki, K.; Li, Y.; Zhu, T.; Wu, J.; Guan, K.-L. : 4: 648-657, 2002. |
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