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| Catalog Code: | NB110-57630 |
| Product Name: | TRPC1 Antibody |
| Product Description: | Rabbit Monoclonal anti-TRPC1 (EP1417Y) |
| Clone: | EP1417Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in human TRPC1 was used as an immunogen. |
| Cross Reactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:10,000FC: 1:50IP: 1:100 |
| Background: | In many vertebrate and invertebrate cells, inositol 1,4,5-trisphospate production induces a biphasic Ca2+ signal. Mobilization of Ca2+ from internal stores drives the initial burst. The second phase occurs when depletion of intracellular Ca2+ pools activates a non-voltage-sensitive plasma membrane Ca2+ conductance (1). TRPC1 (transient receptor potential channel-related protein 1) is a store-operated channel in freshly isolated smooth muscle cells of resistance arterioles, arteries, and veins from human, mouse, or rabbit. TrpC1-specific antibody targeted to peptide predicted to contribute to the outer vestibule of TrpC1 channels revealed that TrpC1 is localized to the plasma membrane and has an extracellular domain. Peptide-specific binding of the antibody had a functional effect, selectively blocking store-operated Ca(2+) channel activity. Studies shows that TRPC1 is a novel physiological Ca(2+) channel subunit in arterial smooth muscle cells (2). It has been reported that the mGluR1-evoked slow EPSC is mediated by the TRPC1 cation channel. TRPC1 is expressed in perisynaptic regions of the cerebellar parallel fibre-Purkinje cell synapse and is physically associated with mGluR1 (3) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu(-), Rt, |
| Alternate Names: | anti-HTRP1 antibody, anti-Short transient receptor potential channel 1 antibody, anti-Transient receptor potential cation channel, subfamily C, member 1 antibody, anti-TRP 1 protein antibody, anti-TRP1 antibody, anti-TRPC1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Wes PD, et al. Proc Natl Acad Sci 92(21):9652-6, 1995. 2. Xu SZ, et al. Circ Res 88(1):84-7, 2001. 3. Kim SJ, et al. Nature 426(6964):285-91, 2003 |
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