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| Catalog Code: | NB110-57624 |
| Product Name: | TpL2 Antibody |
| Product Description: | Rabbit Monoclonal anti-TpL2 (EP1103Y) |
| Clone: | EP1103Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in human Tpl2 was used as an immunogen. |
| Cross Reactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:500 |
| Background: | TPL-2, a MEK kinase is essential for TLR4 activation of the ERK mitogen-activated protein kinase cascade in lipopolysaccharide (LPS)-stimulated macrophages. Interaction with p105 is required to maintain TPL-2 metabolic stability and also negatively regulates TPL-2 MEK kinase activity (1). The NF-kappaB1 gene encodes a larger precursor protein, p105, from which p50 is produced constitutively by proteasome-mediated removal of the p105 carboxy terminus. TPL-2 , which is homologous to MAP-kinase-kinase kinases in its catalytic domain, forms a complex with the carboxy terminus of p105. TPL-2 was originally identified, in a carboxy-terminal-deleted form, as an oncoprotein in rats and is more than 90% identical to the human oncoprotein COT. Expression of TPL-2 results in phosphorylation and increased degradation of p105 while maintaining p50 production. This releases associated Rel subunits or p50-Rel heterodimers to generate active nuclear NF-kappaB. Furthermore, kinase-inactive TPL-2 blocks the degradation of p105 induced by tumor-necrosis factor-alpha. TPL-2 is therefore a component of a new signaling pathway that controls proteolysis of NF-kappaB1 p105 (2). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-Cancer Osaka thyroid oncogene antibody, anti-COT proto oncogene serine/threonine protein kinase antibody, anti-c COT antibody, anti-CCOT antibody, anti-EST antibody, anti-ESTF antibody, anti-Ewing sarcoma transformant antibody, anti-FLJ10486 antibody, anti-MAP3K 8 antibody, anti-Mitogen activated protein kinase kinase kinase 8 antibody, anti-TPL 2 antibody, anti-TPL2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Lang V, et al. Mol Cell Biol 24(12):5235-48, 2004. 2. Belich MP, et al. Nature 397(6717):363-8, 1999. |
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