| CatalogCode: | NB110-57622 |
| ProductName: | Topo II Alpha Phospho (pT1343) Antibody |
| Product Description: | Rabbit Monoclonal anti-Topo II Alpha Phospho (pT1343) (EP1054Y) [Thr1343] |
| Clone: | EP1054Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topo II alpha was used as immunogen. The antibody only detects Topo II alpha phosphorylated on Threonine 1343. |
| CrossReactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1000IHC: 1:100 - 250ICC: 1:50 - 100 |
| Background: | DNA topoisomerase II alpha (Topo IIá) is a 170 kDa ubiquitous nuclear enzyme belonging to the topo family, which regulates the topological states of DNA. Topo II is required in chromatin condensation and segregation during mitosis. Topo II á is cell cycle regulated and its level peaks between G2 to M phase. It has been linked to cell proliferation and it may be the main isoform of Topo II involved mitotic processes (1). Topo II á passes one strand of DBA through a reversible break in a second DNA strand, which catalyzes the topological isomerisation of DNA during cell cycle (2). Topo II á overexpression has been linked to a number of human maligenacies and is the target for many chemotherapeutic agents. The majority of anticancer drugs targeting Topo IIá initiate apoptosis by stabilizing the convalent complex formed between DNA and Topo IIá (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | DNA topoisomerase 2 alpha antibody DNA topoisomerase II 170 kD antibody DNA topoisomerase II alpha isozyme antibody DNA Topoisomerase2 antibody TOP 2A antibody TOP2 antibody TOP2A antibody TopII alpha antibody Topo II alpha antibody Topoisomerase DNA II alpha 170kDa antibody Topoisomerase II alpha 170 kDa antibody TP2A antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Ohasi Y, et al. Anticancer Res, 19:1873-1880, 1999. 2. Sakaguchi A, et al. J Cell Sci 117:1047-104, 2004. 3. Zhou Z, et al. Mol Cancer Res 3:271-275, 2005. |
| Phosphorylated: | Thr1343 |
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