| CatalogCode: | NB110-57611 |
| ProductName: | B-Tubulin, class III (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-B-Tubulin, class III (C-term) (EP1569Y) |
| Clone: | EP1569Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to the C-term of human class III â-Tubulin was used as immunogen. |
| CrossReactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:20,000IHC: 1:50 - 100ICC: 1:100 - 250FC: 1:10IP: 1:40 |
| Background: | Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BS |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | M40 antibody TUBB1 antibody TUBB2 antibody TUBB5 antibody Tubulin beta polypeptide antibody Tubulin beta-2 chain antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Wang, HW et al. Nature 435: 911-915, 2005. 2. Cleveland, DW et al. Cell 20: 95-105, 1980. 3. Hasegawa S et al. Clin Can Res 9: 2992-2997, 2003. |
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