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| Catalog Code: | NB110-57610 |
| Product Name: | B-Tubulin Antibody |
| Product Description: | Rabbit Monoclonal anti-B-Tubulin (EP1331Y) |
| Clone: | EP1331Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues near the C-term of human â-Tubulin was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:20,000IHC: 1:250 - 500ICC: 1:100 - 250FC: 1:10IP: 1:50 |
| Background: | Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BS |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-M(beta)3 antibody, anti-M(beta)6 antibody, anti-MC1R antibody, anti-Tubb 3 antibody, anti-TUBB 4 antibody, anti-Tubb3 antibody, anti-TUBB4 antibody, anti-Tubulin beta 3 antibody, anti-Tubulin beta 3 chain antibody, anti-Tubulin beta 4 antibody, anti-Tubulin beta III antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.25% sodium azide |
| General References: | 1. Wang, HW et al. Nature 435: 911-915, 2005. 2. Cleveland, DW et al. Cell 20: 95-105, 1980. 3. Tsurutani J et al. Lung Cancer 35: 11-16, 2002. |
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