| CatalogCode: | NB110-57604 |
| ProductName: | SYK Antibody |
| Product Description: | Rabbit Monoclonal anti-SYK (EP573Y) |
| Clone: | EP573Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues near the protein kinase domain of human Syk was used as immunogen |
| CrossReactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:5,000-20,000IHC: 1:100-250ICC: 1:100-250 |
| Background: | Syk, a non-receptor tyrosine kinase, belongs to the Syk-Zap70 family of protein tyrosine kinase (PTK) and is involved in a wide variety of cellular functions including the pathogenesis of malignant cancer (1). Ubiquitously expressed in hematopoietic cells, Syk has been found to be an effector of B cell receptor (BCR) and functions in B & T cell lymphopoiesis. In the absence of Syk, B cell development is blocked (2). Upon binding to the BCR and to the cell surface receptor of hematopoietic cells (3), Syk is activated and phosphorylates Phospholipase C resulting in the downstream activation of ERK & JNK kinase. Syk also phosphorylates PI3-K activating the Akt pathway (4-5). Cross-linking of the T cell antigen receptor (TCR)-CD3 complex induces rapid tyrosine phosphorylation and then activation of Syk, which in turn phosphorylates a multitude of intracellular substrates such as Cbl (6). Recent findings reveal that Syk is also expressed in endothelial and epithelial cells, and has tumor-suppressive activity in breast cancer cells (7). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | Spleen tyrosine kinase antibody Tyrosine protein kinase SYK antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Bolen, J. B., J. S. Brugge. 1997. Annu. Rev. Immunol. 15:371 2. Turner M, Schweighoffer E, Colucci F, Di Santo JP, Tybulewicz VL: Immunol Today 2000, 21:148-154. 3. Reth, M.. 1989. Nature 338:383. 4. Jiang A, Craxton A, Kurosaki T, Clark EA: J Exp Med 1998, 188:1297-1306. |
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