| request information: |
|
| Catalog Code: | NB110-57602 |
| Product Name: | Stathmin Antibody |
| Product Description: | Rabbit Monoclonal anti-Stathmin (EP1573Y) |
| Clone: | EP1573Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in C-terminus of human Stathmin was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500,000IHC: 1:250-1:500ICC: 1:100-1:250FC: 1:100IP: 1:50 |
| Background: | Stathmin (oncoprotein 18, op18) is a ubiquitous cytosolic phosphoprotein with various regulatory functions in cell proliferation, differentiation signaling, and activation (1). In particular, stathmin is involved in the regulation of tubulin dynamics through inhibition of microtubule formation and/or microtubule depolymerization. Stathmin interacts with soulable tubulins (á,â-tubulin) resulting in the formation of T2S complex which sequesters free tubulin, impeding tubulin formation (2). Stathmin activity is regulated through phosphorylation at Ser16, Ser25, Ser38 and Ser63 by various protein kinases (e.g. MAP-kinase, p34cdc2 kinase), weakening stathmin's affinity to tubulin (3). A mutation on stathmin can lead to excess build up of mitotic spindle and with possible consequence of unregulated cell cycles seen in cancer cells (4). Stathmin is also known as the generic member of a protein family which includes neural proteins SCG10, SCLIP and RB3/RB3'/RB3. All members in this family exhibits tubulin binding ability. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BS |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Lag antibody, anti-LAP 18 antibody, anti-LAP18 antibody, anti-Leukemia associated phosphoprotein p18 antibody, anti-Metablastin antibody, anti-Oncoprotein 18 antibody, anti-OP 18 antibody, anti-OP18 antibody, anti-p18 antibody, anti-p19 antibody, anti-Phosphoprotein 19 antibody, anti-Phosphoprotein p19 antibody, anti-PP17 antibody, anti-PP19 antibody, anti-PR22 antibody, anti-Pr22 protein antibody, anti-Prosolin antibody, anti-Protein Pr22 antibody, anti-SMN antibody, anti-Stathmin antibody, anti-Stathmin1 antibody, anti-STMN 1 antibody, anti-STMN1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.25% sodium azide |
| General References: | 1. Cassimeris L et al. Curr Opin in Cell Bio. 14:18-24, 2002. 2. Clément MJ et al. Biochemistry. 44:14616-14625, 2005. 3. Jourdain L et al. Biochemistry 36:10817-10821, 1997. 4. Moreno FJ, Avila J, Mol Cell Biochem 183: 201-209, 1998 |
order or
more information: | company product webpage |
|