| CatalogCode: | NB110-57601 |
| ProductName: | STAT5A Antibody |
| Product Description: | Rabbit Monoclonal anti-STAT5A (E289) |
| Clone: | E289 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues in C-terminus of human Stat5-á was used as immunogen. The antibody does not cross-react with other Stat family members. |
| CrossReactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1,000IHC: 1:100-250ICC: 1:100Flow Cytometry: 1:10IP: 1:80 |
| Background: | Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5-á tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the á- and â-isoforms of Stat5 (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.5% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt |
| ALTnames: | Mammary gland factor antibody MGF antibody Signal transducer and activator of transcription 5A antibody STAT 5 antibody STAT 5A antibody STAT5 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Schindler C, Darnell JE Jr. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem. 1995;64:621-651 2. Lin, J.X., et al. Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells. J. Biol Chem. 271: 10738-44 (1996) 3. Litterst, C.M., et al. NCoA-1/SRC-1 is an essential coactivator of STAT5 that binds to the FDL motif in the alpha-helical region of the STAT5 transactivation domain. J Biol Chem. 278: 45340-51 (2003). |
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