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| Catalog Code: | NB110-57598 |
| Product Name: | Stat5 Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-Stat5 (Y694) (E208) [Tyr694] |
| Clone: | E208 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Tyr694 of human Stat5 was used as immunogen. The antibody only detects Stat5 phosphorylated on Tyrosine 694. |
| Cross Reactivity: | Reacts in human and mouse. Does not react in rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000IHC: 1:50ICC: 1:50IP: 1:100 |
| Background: | Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 is tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin (1,2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the a- and b-isoforms of Stat5 (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt(-), |
| Alternate Names: | anti-MGF antibody, anti-Signal Transducer and Activator of Transcription 5A antibody, anti-STAT 5 antibody, anti-STAT 5A antibody, anti-STAT 5B antibody, anti-STAT5 antibody, anti-STAT5A antibody, anti-STAT5B antibody, anti-Transcription factor STAT5A antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Tyr694 |
| General References: | 1. Hou, J., et al. Identification and purification of human Stat proteins activated in response to interleukin-2. Immunity 2: 321-9 (1995). 2. Lin, J.X., et al. Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells. J. Biol Chem. 271: 10738-44 (1996). 3. Litterst, C.M., et al. NCoA-1/SRC-1 is an essential coactivator of STAT5 that binds to the FDL motif in the alpha-helical region of the STAT5 transactivation domain. J Biol Chem. 278: 45340-51 (2003). |
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