| CatalogCode: | NB110-57597 |
| ProductName: | Phospho-Stat3 (pS727) Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-Stat3 (pS727) (E121-31) [Ser727] |
| Clone: | E121-31 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human Stat3 was used as immunogen. The antibody detects Stat3 phosphorylated on Serine 727. It does not cross-react with the phospho-serines of other Stat proteins. |
| CrossReactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:2,000IHC: 1:50ICC: 1:100IP: 1:40 |
| Background: | Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt |
| ALTnames: | Acute Phase Response Factor antibody APRF antibody DNA binding protein APRF antibody FLJ20882 antibody MGC16063 antibody Signal Transductor and Activator of Transcription 3 antibody STAT 3 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Heim, M.H. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19: 75-120 (1999). 2. Kisseleva, T. et al. Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene 285: 1-24 (2002). 3. Bromberg, J.F. et al. Stat3 as an Oncogene. Cell 98: 295-303 (1999). 4. Wen, Z. et al. Maximal activation of transcription by statl and stat3 requires both tyrosine and serine phosphorylation. Cell 82: 241-250 (1995). |
| Phosphorylated: | Ser727 |
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