| CatalogCode: | | NB110-57593 |
| ProductName: | | Src Antibody |
| Product Description: | | Rabbit Monoclonal anti-Src (Y232) [Tyr529] |
| Clone: | | Y232 |
| Clonality: | | Monoclonal |
| Specificity: | | A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 529 of human Src was used as immunogen. The antibody will detect Src phosphorylation on Tyrosine 529 of both isoforms. |
| CrossReactivity: | | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | | 0.1 ml Rabbit ascites. |
| Uses: | | WB: 1:5,000-10,000ICC: 1:50IP: 1:50 |
| Background: | | Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4). |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | | 325 |
| AppSummary: | | IP, WB, ICC |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | ASV antibody c SRC antibody CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody cSrc antibody Neuronal CSRC tyrosine specific protein kinase antibody OTTHUMP00000030931 antibody p60 Src antibody p60Src antibody pp60c src antibody pp60csrc antibody Proto oncogene tyrosine protein kinase Src antibody protooncogene SRC Rous sarcoma antibody Tyrosine kinase pp60c src antibody Tyrosine protein kinase SRC1 antibody v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody vsrc avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | 1. Cooper, J. A., and King, C. S. (1986) Mol. Cell. Biol. 6, 4467-4477 2. Courtneidge, S. A. (1985) EMBO J. 4, 1471-1477 3. Okada, M., Howell, B. W., Broome, M. A., and Cooper, J. A. (1993) J. Biol. Chem. 268, 18070-18075 4. Leiming Li, Masaya Okura, Akira Imamoto Molecular and Cellular Biology, Feb. 2002, p. 1203-1217 |
| Phosphorylated: | | Tyr529 |
| more information: | | company product webpage |
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