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| Catalog Code: | NB110-57584 |
| Product Name: | SMURF2 Antibody |
| Product Description: | Rabbit Monoclonal anti-SMURF2 (EP629Y3) |
| Clone: | EP629Y3 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in human Smurf2 was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500 - 1:1000 |
| Background: | Smad ubiquitination regulatory factor proteins (Smurf1 and Smurf2) are E3 ubiquitin ligase that belongs to the Hect family. Smurf proteins play an important role as regulators in TGF-beta pathway by ubiquitinating Smads and Smads associated proteins for proteasome degradation (1). Specifically, Smurf1 interacts with Smad1 and Smad5 for degradation, while Smurf2 ubiquitinates Smad1 and Smad2. Smads also functions to recruit Smurfs to various pathway components such as TGF-beta and SnoN. In particular, Smad7 acts as an adaptor protein between Smurfs and TGF-Beta receptors, allowing the receptors to be marked by Smurfs for degradation (2). Smurf2 interacts with all members of Smad family except for Smad4 and it is expressed various tissues and cell lines, such as placenta and ovarian cancer cell lines. Smurf2 has been implicated in the tumor formation and diseases progression (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BS |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-EC 6.3.2. antibody, anti-hSMURF2 antibody, anti-MGC138150 antibody, anti-Smad specific E3 ubiquitin ligase 2 antibody, anti-SMAD specific E3 ubiquitin protein ligase 2 antibody, anti-Smad ubiquitination regulatory factor 2 antibody, anti-Ubiquitin protein ligase SMURF2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.25% sodium azide |
| General References: | 1. Hershko A, Ciechanover A, Annu. Rev. Biochem. 67, 425-479. 1988. 2. Ebisawa T et al. J. Biol. Chem. 276:12477-12480, 2001. 3. Cai Y et al. Chin J Dig Dis. 7(4):237-45, 2006. |
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