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| Catalog Code: | NB110-57582 |
| Product Name: | SMAD 4 Antibody |
| Product Description: | Rabbit Monoclonal anti-SMAD 4 (EP618Y) |
| Clone: | EP618Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the C-term of human Smad 4 was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1,000-5,000IHC: 1:100-250ICC: 1:100-250Flow Cytometry: 1:20IP: 1:30 |
| Background: | Smad 4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 4 is the common Smad (co-Smad) (1-2). Previously identified as the tumor suppressor DPC4 (deleted in pancreatic carcinoma, locus 4), Smad 4 is functionally distinct among the Smad family, and is required for the assembly and transcriptional activation of diverse, Smad-DNA complexes (3). In contrast to the R-Smads, Smad 4 is not regulated by phosphorylation, but acts as a common mediator of TGF-â, activin, and bone morphogenetic protein signaling responses (4). Smad 4 is frequently inactivated in pancreatic, biliary and colorectal tumors (5-6). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-(Small) Mothers Against Decapentaplegic antibody, anti-Deleted in Pancreatic Carcinoma antibody, anti-Deleted in pancreatic carcinoma locus 4 antibody, anti-Deletion target in pancreatic carcinoma 4 antibody, anti-DPC 4 antibody, anti-DPC4 antibody, anti-hSMAD4 antibody, anti-JIP antibody, anti-MAD homolog 4 antibody, anti-MAD mothers against decapentaplegic homolog 4 antibody, anti-MADH 4 antibody, anti-MADH4 antibody, anti-Mothers against decapentaplegic homolog 4 antibody, anti-Mothers against DPP homolog 4 antibody, anti-Smad 4 antibody, anti-SMAD family member 4 antibody, anti-SMAD mothers against DPP homolog 4 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Eppert et al. Cell 86: 543-552, 1996 2. Derynck et al.1996.Curr. Biol. 6: 1226-1229 3. Hudson er al. (1998) Development 125, 1407-1420 4. Lagna et al. (1996) Nature 383, 832-836 5. Hahn,et al. (1996) Science 271, 350-353 6. Goggins et al. (1998) Cancer Res. 58, 5329-5332 |
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