| CatalogCode: | NB110-57581 |
| ProductName: | Phospho Smad3 (pS423/425) Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho Smad3 (pS423/425) (EP823Y) [Ser423/Ser425] |
| Clone: | EP823Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic phospho-specific peptide corresponding to residues surrounding Ser423 and Ser425 of human Smad3 was used as an immunogen. The antibody only detects Smad3 phosphorylated on Serine 423 and Serine 425. |
| CrossReactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant . |
| Uses: | WB: 1:2000IHC: 1:100 - 250ICC: 1:100 - 250 |
| Background: | Smad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-â/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-â), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-â receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF- â target genes. Interaction of Smad3 with Akt, can initiate TGF- â induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt |
| ALTnames: | DKFZP586N0721 antibody DKFZp686J10186 antibody hMAD 3 antibody hSMAD3 antibody HSPC193 antibody HST17436 antibody JV15 2 antibody JV152 antibody MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody MAD3 antibody MADH 3 antibody MADH3 antibody MGC60396 antibody Mothers against decapentaplegic homolog 3 antibody Mothers against DPP homolog 3 antibody SMA and MAD related protein 3 antibody SMAD 3 antibody SMAD antibody SMAD3 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Yang X, et al. Devel Biol 95:3667-3672, 1998. 2. Preobrazhenska O, et al. Oncogene 21:5660-5664, 2002. 3. Qing C et al. J Biol Chem 275:38802-38812, 2000. 4. Han SU, et al. Oncogene 23:1333-1341, 2004. |
| Phosphorylated: | Ser423/Ser425 |
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more information: | company product webpage |
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