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| Catalog Code: | NB110-57580 |
| Product Name: | SMAD3 Antibody |
| Product Description: | Rabbit Monoclonal anti-SMAD3 (EP568Y) |
| Clone: | EP568Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to the internal region of human Smad3 was used as immunogen. |
| Cross Reactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant Rabbit ascites. |
| Uses: | WB: 1:5000IHC: 1:100 - 250ICC: 1:100 - 250IP: 1:50 |
| Background: | Smad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-â/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-â), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-â receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF- â target genes. Interaction of Smad3 with Akt, can initiate TGF- â induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt, |
| Alternate Names: | anti-DKFZP586N0721 antibody, anti-DKFZp686J10186 antibody, anti-hMAD 3 antibody, anti-hSMAD3 antibody, anti-HSPC193 antibody, anti-HST17436 antibody, anti-JV15 2 antibody, anti-JV152 antibody, anti-MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody, anti-MAD3 antibody, anti-MADH 3 antibody, anti-MADH3 antibody, anti-MGC60396 antibody, anti-Mothers against decapentaplegic homolog 3 antibody, anti-Mothers against DPP homolog 3 antibody, anti-SMA and MAD related protein 3 antibody, anti-SMAD 3 antibody, anti-SMAD antibody, anti-SMAD3 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Yang X, et al. Devel Biol 95:3667-3672, 1998. 2. Preobrazhenska O, et al. Oncogene 21:5660-5664, 2002. 3. Qing C et al. J Biol Chem 275:38802-38812, 2000. 4. Han SU, et al. Oncogene 23:1333-1341, 2004. |
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