| CatalogCode: | NB110-57579 |
| ProductName: | SMAD2 Antibody |
| Product Description: | Rabbit Monoclonal anti-SMAD2 (EP784Y) |
| Clone: | EP784Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues surrounding the MH2 domain of human Smad2 was used as immunogen. |
| CrossReactivity: | Reacts in human and rat. Does not react in mouse. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500,000IHC: 1:100 - 250ICC: 1:100 - 250IP: 1:50FC: 1:30 |
| Background: | Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt |
| ALTnames: | hMAD 2 antibody hSMAD2 antibody JV18 1 antibody JV18 antibody JV181 antibody MAD antibody MAD Related Protein 2 antibody MADH2 antibody MADR2 antibody MGC22139 antibody MGC34440 antibody Mothers Against Decapentaplegic Homolog 2 antibody mothers against DPP homolog 2 antibody SMAD 2 antibody SMAD antibody SMAD2 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Eppert, et al. Cell 86:543-552, 1996 2. Derynck R, et al. Curr Biol 6:1226-1229, 1996 3. Yagi K, et al. J Biol Chem 274:703-709, 1999 4. Chen X, et al. Nature 383:691-696, 1996 5. Labbé E, et al. Mol Cell 2:109-120, 1998 |
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