| CatalogCode: | NB110-57578 |
| ProductName: | SMAD2 Antibody |
| Product Description: | Rabbit Monoclonal anti-SMAD2 (EP567Y) |
| Clone: | EP567Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues surrounding the MH2 domain of human Smad2 was used as immunogen. |
| CrossReactivity: | Reacts in human. Does not react in mouse or rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500 - 1000IHC: 1:50ICC: 1:250-500Flow Cytometry: 1:20 |
| Background: | Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | hMAD 2 antibody hSMAD2 antibody JV18 1 antibody JV18 antibody JV181 antibody MAD antibody MAD Related Protein 2 antibody MADH2 antibody MADR2 antibody MGC22139 antibody MGC34440 antibody Mothers Against Decapentaplegic Homolog 2 antibody mothers against DPP homolog 2 antibody SMAD 2 antibody SMAD antibody SMAD2 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Eppert et al. Cell 86: 543-552, 1996 2. Derynck, R. and Y. Zhang. 1996. Intracellular signaling: The Mad way to do it. Curr. Biol. 6: 1226-1229 3. Ken Yagi, Daisuke Goto, Toshiaki Hamamoto, Seiichi Takenoshita, Mitsuyasu Kato, and Kohei Miyazono, J Biol Chem, Vol. 274, Issue 2, 703-709, January 8, 1999 4. Chen, X., M.J. Rubock, and M. Whitman. 1996. Nature 383: 691-696 5. Labbé, E., Silvestri, C., Hoodless, P. A., Wrana, J. L., and Attisano, L. (1998) Mol. Cell 2, 109-120 |
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