| CatalogCode: | NB110-57575 |
| ProductName: | SIRT2 Antibody |
| Product Description: | Rabbit Monoclonal anti-SIRT2 (EP1668Y) |
| Clone: | EP1668Y |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide corresponding to residues on the C terminus of human SIR2 / SIRT2. |
| Specificity: | A synthetic peptide corresponding to residues on the C-terminus of human SIRT2 was used as an immunogen. |
| CrossReactivity: | Reacts in human and rat. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant Rabbit ascites. |
| Uses: | WB: 1:1000ICC: 1:100 - 250FC: 1:30IP: 1:50 |
| Background: | Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IP, WB, ICC |
| SpeciesSummary: | Hu, Rt |
| ALTnames: | anti-NAD dependent deacetylase sirtuin 2 antibody, anti-Silencing information regulator 2 like antibody, anti-Silent mating type information regulation 2 antibody, anti-Silent mating type information regulation 2 homolog antibody, anti-SIR 2 antibody, anti-SIR2 like antibody, anti-SIR2 like protein 2 antibody, anti-Sir2 related protein type 2 antibody, anti-SIR2L antibody, anti-SIR2L2 antibody, anti-SIRT 2 antibody, anti-Sirtuin 2 antibody, Sirtuin type 2 antibody, anti-Sirtuin2 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Borra MT, et al. J Biol Chem 277(15):12632-41, 2002. 2. North BJ, et al. Mol Cell 11(2):437-44, 2003. 3. Hiratsuka M, et al. Biochem Biophys Res Commun 309(3):558-66, 2003. |
| Gene_Id: | 22933 |
| Omim_ID: | NB100-2231 |
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